Preamble

BIT-DST sponsored “Laboratory for Venom Peptidomics and Molecular Simulation (LVPMS)” established in 2014 and nested in the Biotechnology department, molecular characterizes toxic peptides and molecules obtained different venomous spies both in land and Sea. State of the art facilities are deployed for obtaining single molecules for therapeutic use. Molecular interactions between the lethal toxins and voltage gated channels with an expertise from both the expertise in International and Indian counterparts aim at developing isoform specific drugs. Various International and National collaboration is involved in this research.

Area of Research

1. Venom Biology
2. Ion Channels and Electrophysiology.
3. Pharmacogenomics.
4. Computational Biology and Bioinformatics.
5. Protein Chemistry and Structural Biology.

Objective

Research

1. Test for the effectiveness of the isolated toxins from conotoxins, Indian spiders and scorpions.
2. To develop a antibody/Toxin/Sodium channel interaction database.
3. Molecular simulation on different modelled channel with toxin structures.
4. Bioinformatic approach to toxin/sodium channel structure-function relationship studies and through known Toxin/Nanobody interaction relationships.
5. To find the selectivity of the toxin with different sodium channel subtypes thereby opening avenue on subtype selective drugs.

Guidance

1. To train manpower by guiding research scholars for research degrees in Toxin and Bioinformatics research.
2. To guide PG and UG students for their final year projects.

LVPMS Research Unit

Principal Investigator

Dr. B A Gowri Shankar, PhD.
Assistant Professor (Sel. Grade)
LVPMS Research Unit,
Department of Biotechnology,
Bannari Amman Institute of Technology,
Alathukombai - Post
Sathyamangalam - 638 401
Erode District, Tamil Nadu, India
Ph: +91-4295-226256
Fax: +91-4295-226666
E-mail: arungowrishankar@gmail.com / gowrishankarba@bitsathy.ac.in

PhD Students

  1. Ms. A M Priyadharsini, M.Tech, JRF, Indo-Tunisian funded Project.
  2. Ms. Arathrika Sarkar

Collaborators

National

Prof. P Gautam, Director and Head, Centre for Biotechnology, Anna University, Chennai.

International
Prof. Rosa Lemmens Gruber,  Associate Professor,  Deartment of pharmacology and Toxicology, University of Vienna, Austria.
Prof. Balkiss Bouhaouala-Zahar, Professor of Biochemistry, Institut Pasteur Tunis, Tunisia
Dr Rym Benkhalifa, Institut Pasteur Tunis, Tunisia
Dr. Igor Križaj, Professor, Department of Molecular and Biomedical Sciences, Jožef Stefan Institute, Ljubljana, Slovenia

Funded Ongoing Projects

Indo-Tunisian Project Jointly sponsored by Dept. of Science & Technology, Govt. of India and Ministry of Higher Education and Scientific Research of the Republic of Tunisia

Title : Toxin and voltage gated sodium channel interactions: A study on structure-activity relationships
Indian PI: Dr. B A Gowri Shankar, BIT
Tunisian PI: Prof. Balkiss  Bouhaouala-Zahar,  Institut Pasteur Tunis,
Sanction No. : INT/Tunisia/P-06/2013, 04-06-2013
Budget: INR 29,01,000
Time Period: 2013 - 2016


Indo-Austrian Project Jointly sponsored by Dept. of Science & Technology, Govt. of India and Austrian Science Fund (FWF), Govt. of Austria.

Title : Toxin and voltage gated sodium channel interactions: a  bioinformatic approach of  structure-activity relationships
Indian PI: Dr. B A Gowri Shankar, BIT>
Tunisian PI: Prof. Rosa Lemmens Gruber, University of Vienna, Austria.
Sanction No. : INT/AUA /BMWF/P-14/2013, 04-06-2013 Budget: INR 3,82,100
Time Period: 2013 – 2015

Publications

Articles in journals with peer review
  1. Andavan G.S.B., Lemmens-Gruber R. Voltage Gated Sodium Channels: mutations, channelopathies and subtype specific targets. 2011, Current Medicinal Chemistry, 18(3), 377-97.
  2. Hazemi P., Tzotzos S.J., Fischer B., Andavan G.S.B., Fischer H., Pietschmann H., Lucas R., Lemmens-Gruber R. Essential structural features of TNF-α lectin-like domain derived peptides for activation of amiloride-sensitive sodium current in A549 cells.2010, Journal of Medicinal Chemistry, 53(22), 8021-9.
  3. Andavan G.S.B., Lemmens-Gruber R.Cyclodepsipeptides from marine sponges: natural probe for molecular cell biology. 2010, Marine Drugs, 8(3), 8210-834.
  4. Andavan G.S.B., Sarani R., Michael D., Mridula P., Ranjani C.V., Sowmiya G., Vasundhar B., Sudha P., Jeyakanthan J., Velmurugan D., Sekar K. Ion-pairs in non-redundant protein structures. 2007, Journal of Biosciences, 32(4), 693-704.

Manuscript submitted
  1. Andavan G.S.B., Lemmens-Gruber R. Modulation of cardiac voltage gated sodium channel variants by Fyn tyrosine kinase. (Submitted to Journal of Biological Chemistry)
Proceedings of symposia
  1. Gowri Shankar B.A., Charleston M., Murray M., Hibbs D. A Structural and Phylogenetic Analysis of Cytochrome P450. 2007, International Proceedings of the 15th International Conference on Cytochrome P450: Biochemistry, Biophysics, Functional Genomics, 75-80.
  2. Andavan G.S.B., Lemmens-Gruber R. Intracellular loop connecting domain II and III in the modulation of cardiac voltage gated sodium channel by Fyn tyrosine kinase. 2011, Proceedings (N628) of the XXX Annual Meeting of the European Section of the ISHR (June 26-29, 2011, Haifa, Israel), N628C0009.
Conference Abstracts
  1. Andavan G.S.B., Lemmens-Gruber R. Modulation of cardiac voltage gated sodium channel splice variants with its auxiliary subunit by Fyn tyrosine kinase. 2010, European Heart Journal, 31 (Abstract Supplement), 697.
  2. Andavan G.S.B., Lemmens-Gruber R. Cardiac sodium channel variants and fyn tyrosine kinase modulation.2010, Cardiovascular Research, 87 (Suppl 1):S106.
  3. Andavan G.S.B., Lemmens-Gruber R. Fyn tyrosine kinase modulation on cardiac voltage gated sodium channel splice variants. 2010, ISHR World Congress: Paradigm Shift to Integrated Cardiology -Gene, Function and Life, 1(1), May 13-16, Kyoto International Conference Center, Kyoto, Japan.
  4. Andavan G.S.B., Lemmens-Gruber R. Modulation of Nav1.5 variants by src tyrosine kinase. 2010, Biophysical journal, 310a.
  5. Andavan G.S.B., Charleston M., Murray M., Hibbs D. A Structural and Phylogenetic analysis of Cytochrome P450. 2007, 15th International Conference on Intelligent System for Molecular Biology (ISMB) Jointly with European Conference on Computational Biology (ECCB), M24, 21-25 July, Austria Centre, Vienna, Austria,.
  6. Andavan G.S.B., Vasundhar B., Sekar K. Ion-Pair in Non-Redundant Protein Structures. & Andavan G.S.B., Charleston M., Murray M., Hibbs D. Cytochrome P450 Structures - Structural and Phylogenetic analysis. 2007, 11th Annual International Conference on Research in Computational Molecular Biology (RECOMB), 21-25 April, San Francisco, USA.

Professional Society Members

  1. Biophysical Society (Since 2009).
  2. International Society of Heart Research (Since 2009).
  3. European Society of Cardiology (Since 2010).
  4. Working Group on Cardiac Cellular Electrophysiology of the ESC.